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KMID : 0811720110150060383
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Korean Journal of Physiology & Pharmacology
2011 Volume.15 No. 6 p.383 ~ p.388
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Role of Regulators of G-Protein Signaling 4 in Ca2＋ Signaling in Mouse Pancreatic Acinar Cells
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:Park Soon-Hong
:Lee Syng-Ill/:Lee Syng-Ill/:Shin Dong-Min
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Abstract
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Regulators of G-protein signaling (RGS) proteins are regulators of Ca2＋ signaling that accelerate the GTPase activity of the G-protein ?-subunit. RGS1, RGS2, RGS4, and RGS16 are expressed in the pancreas, and RGS2 regulates G-protein coupled receptor (GPCR)-induced Ca2＋ oscillations. However, the role of RGS4 in Ca2＋ signaling in pancreatic acinar cells is unknown. In this study, we investigated the mechanism of GPCR-induced Ca2＋ signaling in pancreatic acinar cells derived from RGS4?/? mice. RGS4?/? acinar cells showed an enhanced stimulus intensity response to a muscarinic receptor agonist in pancreatic acinar cells. Moreover, deletion of RGS4 increased the frequency of Ca2＋ oscillations. RGS4?/? cells also showed increased expression of sarco/endoplasmic reticulum Ca2＋ ATPase type 2. However, there were no significant alterations, such as Ca2＋ signaling in treated high dose of agonist and its related amylase secretion activity, in acinar cells from RGS4?/? mice. These results indicate that RGS4 protein regulates Ca2＋ signaling in mouse pancreatic acinar cells.
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KEYWORD
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RGS4, Ca2＋ signaling, Pancreatic acinar cells
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